Genetic Requirements of Phage λ Red-Mediated Gene Replacement in Escherichia coli K-12
نویسندگان
چکیده
منابع مشابه
Exonuclease requirements for recombination of λ phage in recD mutants of Escherichia coli
Recombination of λ red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5’-3’ exonuclease activity of RecJ and exonuclease VII is essential for λ recombination, whereas exonucleases of 3’-5’ polarity are dispensable. The implications of the presen...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2000
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.182.8.2336-2340.2000